TY - JOUR
T1 - Genetic analysis of hepatitis C virus with defective genome and its infectivity in vitro
AU - Sugiyama, Kazuo
AU - Suzuki, Kenji
AU - Nakazawa, Takahide
AU - Funami, Kenji
AU - Hishiki, Takayuki
AU - Ogawa, Kazuya
AU - Saito, Satoru
AU - Shimotohno, Kumiko W.
AU - Suzuki, Takeshi
AU - Shimizu, Yuko
AU - Tobita, Reiri
AU - Hijikata, Makoto
AU - Takaku, Hiroshi
AU - Shimotohno, Kunitada
PY - 2009/7
Y1 - 2009/7
N2 - Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.
AB - Replication and infectivity of hepatitis C virus (HCV) with a defective genome is ambiguous. We molecularly cloned 38 HCV isolates with defective genomes from 18 patient sera. The structural regions were widely deleted, with the 5′ untranslated, core, and NS3-NS5B regions preserved. All of the deletions were in frame, indicating that they are translatable to the authentic terminus. Phylogenetic analyses showed self-replication of the defective genomes independent of full genomes. We generated a defective genome of chimeric HCV to mimic the defective isolate in the serum. By using this, we demonstrated for the first time that the defective genome, as it is circulating in the blood, can be encapsidated as an infectious particle by trans complementation of the structural proteins.
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U2 - 10.1128/JVI.02674-08
DO - 10.1128/JVI.02674-08
M3 - Article
C2 - 19369330
AN - SCOPUS:67449099426
SN - 0022-538X
VL - 83
SP - 6922
EP - 6928
JO - Journal of Virology
JF - Journal of Virology
IS - 13
ER -
