Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Shinya Kaneko; Takashi Takeuchi; Mitsuhiro Itaya

doi:10.1016/j.jbiotec.2008.12.007

Genetic connection of two contiguous bacterial artificial chromosomes using homologous recombination in Bacillus subtilis genome vector

Shinya Kaneko, Takashi Takeuchi, Mitsuhiro Itaya

環境情報学部

研究成果: Article › 査読

12 被引用数 (Scopus)

抄録

A Bacillus subtilis genome (BGM) vector system using homologous recombination was applied to connect two contiguous BAC clones covering the entire 355-kb transcription unit of the mouse jumonji genomic region. Results from the convenient genomic manipulation indicated that the BGM system facilitates the connection of DNAs from a BAC library without exchange and deletion of original sequence, which can expand large-sized DNA construction beyond BAC-building in Escherichia coli.

本文言語	English
ページ（範囲）	211-213
ページ数	3
ジャーナル	Journal of Biotechnology
巻	139
号	3
DOI	https://doi.org/10.1016/j.jbiotec.2008.12.007
出版ステータス	Published - 2009 2月 5

ASJC Scopus subject areas

バイオテクノロジー
バイオエンジニアリング
応用微生物学とバイオテクノロジー

文献へのアクセス

10.1016/j.jbiotec.2008.12.007

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引用スタイル

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