Genetic transfer of large DNA inserts to designated Loci of the Bacillus subtilis 168 genome

Mitsuhiro Itaya

研究成果: Article査読

19 被引用数 (Scopus)

抄録

It was found that contiguous DNA segments of up to 50 kb can be transferred between Bacillus subtilis genomes when a sufficient length of the flanking genomic region is provided for homologous recombination, although the efficiency of transfer was reduced as the insert size increased. Inserts were translocated to different loci, where appropriate integration sites were created.

本文言語English
ページ(範囲)1045-1048
ページ数4
ジャーナルJournal of Bacteriology
181
3
DOI
出版ステータスPublished - 1999 2月
外部発表はい

ASJC Scopus subject areas

  • 微生物学
  • 分子生物学

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