Genome-wide identification of endothelial cell-enriched genes in the mouse embryo

Haruka Takase, Ken Matsumoto, Rie Yamadera, Yoshiaki Kubota, Ayaka Otsu, Rumiko Suzuki, Hiroyuki Ishitobi, Hiromi Mochizuki, Takahiro Kojima, Shingo Takano, Kazuhiko Uchida, Satoru Takahashi, Masatsugu Ema

研究成果: Article査読

48 被引用数 (Scopus)


The early blood vessels of the embryo and yolk sac in mammals develop by aggregation of de novo - forming angioblasts into a primitive vascular plexus, which then undergoes a complex remodeling process. Angiogenesis is also important for disease progression in the adult. However, the precise molecular mechanism of vascular development remains unclear. It is therefore of great interest to determine which genes are specifically expressed in developing endothelial cells (ECs). Here, we used Flk1-deficient mouse embryos, which lack ECs, to perform a genome-wide survey for genes related to vascular development. We identified 184 genes that are highly enriched in developing ECs. The human orthologs of most of these genes were also expressed in HUVECs, and small interfering RNA knockdown experiments on 22 human orthologs showed that 6 of these genes play a role in tube formation by HUVECs. In addition, we created Arhgef15 knockout and RhoJ knockout mice by a genetargeting method and found that Arhgef15 and RhoJ were important for neonatal retinal vascularization. Thus, the genes identified in our survey show high expression in ECs; further analysis of these genes should facilitate our understanding of the molecular mechanisms of vascular development in the mouse.

出版ステータスPublished - 2012 7月 26

ASJC Scopus subject areas

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学


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