@article{c118226cc082488e955e960c151bde7b,
title = "H1foo has a pivotal role in qualifying induced pluripotent stem cells",
abstract = "Embryonic stem cells (ESCs) are a hallmark of ideal pluripotent stem cells. Epigenetic reprogramming of induced pluripotent stem cells (iPSCs) has not been fully accomplished. iPSC generation is similar to somatic cell nuclear transfer (SCNT) in oocytes, and this procedure can be used to generate ESCs (SCNT-ESCs), which suggests the contribution of oocyte-specific constituents. Here, we show that the mammalian oocyte-specific linker histone H1foo has beneficial effects on iPSC generation. Induction of H1foo with Oct4, Sox2, and Klf4 significantly enhanced the efficiency of iPSC generation. H1foo promoted in vitro differentiation characteristics with low heterogeneity in iPSCs. H1foo enhanced the generation of germline-competent chimeric mice from iPSCs in a manner similar to that for ESCs. These findings indicate that H1foo contributes to the generation of higher-quality iPSCs.",
author = "Akira Kunitomi and Shinsuke Yuasa and Fumihiro Sugiyama and Yuki Saito and Tomohisa Seki and Dai Kusumoto and Shin Kashimura and Makoto Takei and Shugo Tohyama and Hisayuki Hashimoto and Toru Egashira and Yoko Tanimoto and Saori Mizuno and Shoma Tanaka and Hironobu Okuno and Kazuki Yamazawa and Hideo Watanabe and Mayumi Oda and Ruri Kaneda and Yumi Matsuzaki and Toshihiro Nagai and Hideyuki Okano and Yagami, {Ken Ichi} and Mamoru Tanaka and Keiichi Fukuda",
note = "Funding Information: Nanog-GFP-IRES-puro transgenic mice were kindly provided from Dr. Shinya Yamanaka. Several ESCs were kindly donated: R1 ESC from Dr. John C. Roder, B6J-23 ˆ(UTR) ESC from Dr. Fumihiro Sugiyama, and SCNT-ESC (B6mt-1) from Riken BioResource Center. This study was supported in part by research grants from Grants-in-Aid for Scientific Research (JSPS KAKENHI grant numbers 24117716 , 26670408 , 15H01521 , 15K14431 ), the Highway Program for Realization of Regenerative Medicine from Japan Science and Technology Agency , the Program for Intractable Diseases Research utilizing Disease-specific iPSCs from the Japan Agency For Medical Research and Development (AMED), Translational Research Network Program from AMED , and Keio University Medical Science Fund. H.O. is a Founding Scientist and a paid SAB of San Bio Co. Ltd. Publisher Copyright: {\textcopyright} 2016 The Author(s).",
year = "2016",
month = jun,
day = "14",
doi = "10.1016/j.stemcr.2016.04.015",
language = "English",
volume = "6",
pages = "825--833",
journal = "Stem cell reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "6",
}