TY - GEN
T1 - Handheld nanopore-based biosensing device
AU - Izawa, Yusuke
AU - Osaki, Toshihisa
AU - Kamiya, Koki
AU - Fujii, Satoshi
AU - Misawa, Nobuo
AU - Miki, Norihisa
AU - Takeuchi, Shoji
N1 - Funding Information:
This work was partly supported by KAKENHI (JP17H02758), JSPS, Strategic Advancement of MultiPurpose Ultra-Human Robot and Artificial Intelligence Technologies Project of NEDO, and the Regional Innovation Strategy Support Program of MEXT, Japan. The authors thank to Mses. Uchida and Hashino (KISTEC) for their kind assistance.
Publisher Copyright:
© 2018 IEEE.
PY - 2018/4/24
Y1 - 2018/4/24
N2 - This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.
AB - This paper describes the development of a handheld device for long-term nanopore-based biosensing. Recently, membrane protein reconstituted in bilayer lipid membrane (BLM) has been applied for bio/chemical sensors because membrane protein intrinsically has a superior amplification mechanism. For mobile use of such devices, however, the bilayer requires mechanical robustness. Here, we propose a handheld BLM device aiming for a nanopore-based mobile sensor. BLM is formed at the tip of a glass capillary, which is contained in the solution trapped in a cup-like reservoir. This format achieved (i) formation of a sufficiently robust BLM for mobile use, (ii) solution exchange without rupturing BLM, and (iii) sustaining the small number of nanopores in the bilayer for accurate and long-term sensing. The handheld device successfully detected DNA translocations through the nanopores continuously for 75 minutes using 5 nanopores.
UR - http://www.scopus.com/inward/record.url?scp=85046997151&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046997151&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2018.8346466
DO - 10.1109/MEMSYS.2018.8346466
M3 - Conference contribution
AN - SCOPUS:85046997151
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 2
EP - 5
BT - 2018 IEEE Micro Electro Mechanical Systems, MEMS 2018
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 31st IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2018
Y2 - 21 January 2018 through 25 January 2018
ER -