Hematopoietic activity of tek-ligand, angiopoietins in murine bone marrow cells

Xu Ling Huang, Nobuyuki Takakura, Toshio Suda

研究成果: Article査読


TEK is a receptor tyrosine kinase specific to vascular endothelial cells and hematopoietic cells. It plays critical roles in vascular maturation, integrity and remodeling, and also in the development of definitive hematopoiesis in AGM region. To characterize TEK function in the bone marrow hematopoiesis, we examined the effect of two recently identified TEK-ligands, Angiopoietin-1 and -2 (which were provided by Dr. Yancopoulos, NY). Angiopoietin-1, as a physiologically predominant ligand, effectively induced phosphrylation of TEK and adhesion of TEK+ cells to fibronectin and the adhesive effect was blocked by ROD peptide. Angiopoietins supported the proliferation of hematopoietic cells in the culture of total marrow cells, and this effect was blocked by TEK-Fc fusion protein or ROD. Angiopoietins alone did not affect the proliferation of PECAM-1 + endothelial cells, but by the addition of VEGF, vascular network formation was synergistically enhanced. Angiopoietin-1 stimulated the proliferation of sorted TEK+ cells in the presence of SCF and IL-6. TEK-Fc was intraperitoneally injected into murine neonates. tissue sections and flow cytometric analysis revealed that hematopoietic progenitors including Lin"Kit+ cells significantly reduced. Our findings suggest that TEK signaling is essential to the adherence and proliferation of hematopoietic cells.

ジャーナルExperimental Hematology
出版ステータスPublished - 1998 12月 1

ASJC Scopus subject areas

  • 分子生物学
  • 血液学
  • 遺伝学
  • 細胞生物学
  • 癌研究


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