Heme controls the structural rearrangement of its sensor protein mediating the hemolytic bacterial survival

Megumi Nishinaga, Hiroshi Sugimoto, Yudai Nishitani, Seina Nagai, Satoru Nagatoishi, Norifumi Muraki, Takehiko Tosha, Kouhei Tsumoto, Shigetoshi Aono, Yoshitsugu Shiro, Hitomi Sawai

研究成果: Article査読

7 被引用数 (Scopus)

抄録

Hemes (iron-porphyrins) are critical for biological processes in all organisms. Hemolytic bacteria survive by acquiring b-type heme from hemoglobin in red blood cells from their animal hosts. These bacteria avoid the cytotoxicity of excess heme during hemolysis by expressing heme-responsive sensor proteins that act as transcriptional factors to regulate the heme efflux system in response to the cellular heme concentration. Here, the underlying regulatory mechanisms were investigated using crystallographic, spectroscopic, and biochemical studies to understand the structural basis of the heme-responsive sensor protein PefR from Streptococcus agalactiae, a causative agent of neonatal life-threatening infections. Structural comparison of heme-free PefR, its complex with a target DNA, and heme-bound PefR revealed that unique heme coordination controls a >20 Å structural rearrangement of the DNA binding domains to dissociate PefR from the target DNA. We also found heme-bound PefR stably binds exogenous ligands, including carbon monoxide, a by-product of the heme degradation reaction.

本文言語English
論文番号467
ジャーナルCommunications biology
4
1
DOI
出版ステータスPublished - 2021 12月
外部発表はい

ASJC Scopus subject areas

  • 医学(その他)
  • 生化学、遺伝学、分子生物学一般
  • 農業および生物科学一般

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