Hes1-deficient mice show precocious differentiation of Paneth cells in the small intestine

Katsumasa Suzuki, Hirokazu Fukui, Takahisa Kayahara, Mitsutaka Sawada, Hiroshi Seno, Hiroshi Hiai, Ryoichiro Kageyama, Hideyuki Okano, Tsutomu Chiba

研究成果: Article査読

65 被引用数 (Scopus)


We have previously shown that Hes1 is expressed both in putative epithelial stem cells just above Paneth cells and in the crypt base columnar cells between Paneth cells, while Hes1 is completely absent in Paneth cells. This study was undertaken to clarify the role of Hes1 in Paneth cell differentiation, using Hes1-knockout (KO) newborn (P0) mice. Electron microscopy revealed premature appearance of distinct cells containing cytoplasmic granules in the intervillous region in Hes1-KO P0 mice, whereas those cells were absent in wild-type (WT) P0 mice. In Hes1-KO P0 mice, the gene expressions of cryptdins, exclusively present in Paneth cells, were all enhanced compared with WT P0 mice. Immunohistochemistry demonstrated increased number of both lysozyme-positive and cryptdin-4-positive cells in the small intestinal epithelium of Hes1-KO P0 mice as compared to WT P0 mice. Thus, Hes1 appears to have an inhibitory role in Paneth cell differentiation in the small intestine.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 2005 3月 4

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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