Highly activatable and environment-insensitive optical highlighters for selective spatiotemporal imaging of target proteins

Tomonori Kobayashi, Toru Komatsu, Mako Kamiya, Cláudia Campos, Marcos González-Gaitán, Takuya Terai, Kenjiro Hanaoka, Tetsuo Nagano, Yasuteru Urano

研究成果: Article査読

108 被引用数 (Scopus)

抄録

Optical highlighters are photoactivatable fluorescent molecules that exhibit pronounced changes in their spectral properties in response to irradiation with light of a specific wavelength and intensity. Here, we present a novel design strategy for a new class of caged BODIPY (4,4-difluoro-4-bora-3a, 4a-diaza-s-indacene) fluorophores, based on the use of photoremovable protecting groups (PRPGs) with high reduction potentials that serve as both a photosensitive unit and a fluorescence quencher via photoinduced electron transfer (PeT). 2,6-Dinitrobenzyl (DNB)-caged BODIPY was efficiently photoactivated, with activation ratios exceeding 600-fold in aqueous solutions. We then combined this photoactivatable fluorophore with a SNAP (mutant of O 6-alkylguanine DNA alkyltransferase) ligand to obtain a small-molecule-based optical highlighter for visualization of protein dynamics, using the well-established SNAP tag technology. As proof of concept, we demonstrate spatiotemporal imaging of the fusion protein of epidermal growth factor receptor (EGFR) with SNAP tag in living cells. We also demonstrate highlighting of cells of interest in live zebrafish embryos, using the fusion protein of histone 2A with SNAP tag.

本文言語English
ページ(範囲)11153-11160
ページ数8
ジャーナルJournal of the American Chemical Society
134
27
DOI
出版ステータスPublished - 2012 7月 11
外部発表はい

ASJC Scopus subject areas

  • 触媒
  • 化学一般
  • 生化学
  • コロイド化学および表面化学

フィンガープリント

「Highly activatable and environment-insensitive optical highlighters for selective spatiotemporal imaging of target proteins」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル