Highly Sensitive Bioluminescent Probe for Thiol Detection in Living Cells

Mayu Hemmi, Yuma Ikeda, Yutaka Shindo, Takahiro Nakajima, Shigeru Nishiyama, Kotaro Oka, Moritoshi Sato, Yuki Hiruta, Daniel Citterio, Koji Suzuki

研究成果: Article査読

21 被引用数 (Scopus)


The sensitive detection of thiols including glutathione and cysteine is desirable owing to their roles as indispensable biomolecules in maintaining intracellular biological redox homeostasis. Herein, we report the design and synthesis of SEluc-1 (sulfinate ester luciferin), a chemoselective probe exhibiting a ratiometric and turn-on response towards thiols selectively in fluorescence and bioluminescence, respectively. The probe, which was designed based on the “caged” luciferin strategy, displays excellent selectivity, high signal/noise ratio (>240 in the case of bioluminescence), and a biologically relevant limit of detection (LOD, 80 nm for cysteine), which are all desirable traits for a sensitive bioluminescent sensor. SEluc-1 was further applied to fluorescence imaging of thiol activity in living human cervical cancer HeLa cell cultures, and was successfully able to detect fluctuations in thiol concentrations induced by oxidative stress in a bioluminescent assay utilizing African green monkey fibroblast COS-7 cells and human breast adenocarcinoma MCF-7 cells.

ジャーナルChemistry - An Asian Journal
出版ステータスPublished - 2018 3月 16

ASJC Scopus subject areas

  • 化学 (全般)
  • 生化学
  • 有機化学


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