Highly sensitive visual restoration and protection via ectopic expression of chimeric rhodopsin in mice

Yusaku Katada, Kazuho Yoshida, Naho Serizawa, Deokho Lee, Kenta Kobayashi, Kazuno Negishi, Hideyuki Okano, Hideki Kandori, Kazuo Tsubota, Toshihide Kurihara

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Photoreception requires amplification by mammalian rhodopsin through G protein activation, which requires a visual cycle. To achieve this in retinal gene therapy, we incorporated human rhodopsin cytoplasmic loops into Gloeobacter rhodopsin, thereby generating Gloeobacter and human chimeric rhodopsin (GHCR). In a murine model of inherited retinal degeneration, we induced retinal GHCR expression by intravitreal injection of a recombinant adeno-associated virus vector. Retinal explant and visual thalamus electrophysiological recordings, behavioral tests, and histological analysis showed that GHCR restored dim-environment vision and prevented the progression of retinal degeneration. Thus, GHCR may be a potent clinical tool for the treatment of retinal disorders.

本文言語English
論文番号107716
ジャーナルiScience
26
10
DOI
出版ステータスPublished - 2023 10月 20

ASJC Scopus subject areas

  • 一般

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