Hippo pathway controls cell adhesion and context dependent cell competition to influence skin engraftment efficiency

Miki Nishio, Yousuke Miyachi, Junji Otani, Shoji Tane, Hirofumi Omori, Fumihito Ueda, Hideru Togashi, Takehiko Sasaki, Tak Wah Mak, Kazuwa Nakao, Yasuyuki Fujita, Hiroshi Nishina, Tomohiko Maehama, Akira Suzuki

研究成果: Article査読

10 被引用数 (Scopus)


Cell competition is involved in mammalian embryogenesis and tumor elimination and progression. It was previously shown that, whereas NIH3T3 mouse fibroblasts expressing high levels of the yes-associated protein 1(YAP1) target TEA domain family (TEAD) transcription factors become “winners” in cell competitions, Madin-Darby canine kidney cells expressing activated YAP1 become “losers” and are eliminated from culture monolayers. Thus, YAP1’s role in cell competitions is clearly context dependent. Here, we show that keratinocytes overexpressing a constitutively activated YAP1 mutant lose in in vitro competitions with control cells conducted in standard tissue culture dishes and undergo apical extrusion. Similarly, cells in which endogenous YAP1 is activated by NF2 knockdown become losers. The YAP1-overexpressing cells exhibit a decrease in cell-matrix adhesion because of defective expression of adhesion molecules such as fibronectin-1. Cell adhesion–mediated proliferation is also impaired. However, because of intrinsic factors, YAP1-expressing cells proliferate faster than control cells when cocultured in dishes impeding cell adhesion. In vivo, Mob1a/b-deficient (YAP1-activated) epidermis, which shows decreased expression of type XVII collagen, cannot be engrafted successfully onto donor mice. YAP1-activated skin grafts shrink away from surrounding control skin, and the epidermis peels off the basement membrane. Our data show that YAP1 activation controls cell competition in part by decreasing cell adhesion.

ジャーナルFASEB Journal
出版ステータスPublished - 2019

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生化学
  • 分子生物学
  • 遺伝学


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