Histone acetylation and subcellular localization of chromosomal protein BRD4 during mouse oocyte meiosis and mitosis

Takashi Nagashima, Tetsuo Maruyama, Masataka Furuya, Takashi Kajitani, Hiroshi Uchida, Hirotaka Masuda, Masanori Ono, Toru Arase, Keiko Ozato, Yasunori Yoshimura

研究成果: Article査読

27 被引用数 (Scopus)


Most specific and general transcription factors (TFs) become dissociated from hypoacetylated mitotic chromosomes, which may contribute to transcriptional silencing during mitosis. Only some chromosomal proteins, such as bromodomain containing protein 4 (BRD4), have a potential to associate with mitotic chromosomes in a histone acetylation-dependent manner. It remains to be fully demonstrated whether similar displacement of nuclear factors takes place in meiotic oocytes whose chromosomes become globally deacetylated. To address this, we here examined the subcellular localization of BRD4 in conjunction with the acetylation status of histones in mouse oocytes. Immunofluorescence studies revealed that BRD4 preferentially localized to mitotic chromosomes in early embryos. In contrast, not only endogenous BRD4 but also exogenous BRD4 overexpressed by mRNA microinjection were displaced from meiotic chromosomes whose histones H3 and H4 were deacetylated. Treatment with trichostatin A (TSA), an inhibitor of histone deacetylases, induced histone hyperacetylation of meiotic chromosomes from which endogenous BRD4, however, remained dissociated. Finally, meiotic chromosomal localization of BRD4 could be achieved by BRD4 overexpression together with TSA-induced histone hyperacetylation. These results indicate that, unlike mitosis, histone acetylation is necessary but not sufficient for chromosomal localization of BRD4 during meiosis, suggesting that meiotic oocytes may have additional mechanism(s) for displacement of chromosomal proteins and TFs.

ジャーナルMolecular Human Reproduction
出版ステータスPublished - 2007 3月

ASJC Scopus subject areas

  • 生殖医学
  • 胎生学
  • 分子生物学
  • 遺伝学
  • 産婦人科学
  • 発生生物学
  • 細胞生物学


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