Homogenization of initial cell distribution by secondary flow of medium improves cell culture efficiency

Yuki Fukuma, Takumi Inui, Chikahiro Imashiro, Yuta Kurashina, Kenjiro Takemura

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Homogenization of the initial cell distribution is essential for effective cell development. However, there are few previous reports on efficient cell seeding methods, even though the initial cell distribution has a large effect on cell proliferation. Dense cell regions have an inverse impact on cell development, known as contact inhibition. In this study, we developed a method to homogenize the cell seeding density using secondary flow, or Ekman transportation, induced by orbital movement of the culture dish. We developed an orbital shaker device that can stir the medium in a 35-mm culture dish by shaking the dish along a circular orbit with 2 mm of eccentricity. The distribution of cells in the culture dish can be controlled by the rotational speed of the orbital shaker, enabling dispersion of the initial cell distribution. The experimental results indicated that the cell density became most homogeneous at 61 rpm. We further evaluated the cell proliferation after homogenization of the initial cell density at 61 rpm. The results revealed 36% higher proliferation for the stirred samples compared with the non-stirred control samples. The present findings indicate that homogenization of the initial cell density by Ekman transportation contributes to the achievement of higher cell proliferation.

本文言語English
論文番号e0235827
ジャーナルPloS one
15
7 July
DOI
出版ステータスPublished - 2020 7月

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学一般
  • 農業および生物科学一般
  • 一般

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