TY - JOUR
T1 - Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine
AU - Kawai, Narudo
AU - Ogura, Yosuke
AU - Ikuta, Tetsuro
AU - Saiga, Hidetoshi
AU - Hamada, Mayuko
AU - Sakuma, Tetsushi
AU - Yamamoto, Takashi
AU - Satoh, Nori
AU - Sasakura, Yasunori
N1 - Funding Information:
We would like to thank the members of the Shimoda Marine Research Center at the University of Tsukuba for their assistance with this study. We would also like to thank Drs. Shigeki Fujiwara, Nobuo Yamaguchi, Kunifumi Tagawa and all members of the Department of Zoology, Kyoto University, Misaki Marine Biological Station, University of Tokyo, and the Maizuru Fishery Research Station of Kyoto University for collecting Ciona adults. Drs. Patrick Lemaire and Agnes Roure are acknowledged for their kind provision of Full ORF gateway compatible clones. We thank Dr. Steven Aird for English editing of the manuscript. This study was supported by Grants-in-Aid for Scientific Research from JSPS and MEXT ( 20681019, 21112004, 23681039 ) and Innovative Research Support Program (Pilot Models) from University of Tsukuba to YS. Further support was provided by Grants from the National Bioresource Project . We also thank OIST Graduate University for its generous support of the Marine Genomics Unit.
Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/7/1
Y1 - 2015/7/1
N2 - Hox cluster genes play crucial roles in development of the metazoan antero-posterior axis. Functions of Hox genes in patterning the central nervous system and limb buds are well known. They are also expressed in chordate endodermal tissues, where their roles in endodermal development are still poorly understood. In the invertebrate chordate, Ciona intestinalis, endodermal tissues are in a premature state during the larval stage, and they differentiate into the digestive tract during metamorphosis. In this study, we showed that disruption of a Hox gene, Ci-Hox10, prevented intestinal formation. Ci-Hox10-knock-down larvae displayed defective migration of endodermal strand cells. Formation of a protrusion, which is important for cell migration, was disrupted in these cells. The collagen type IX gene is a downstream target of Ci-Hox10, and is negatively regulated by Ci-Hox10 and a matrix metalloproteinase ortholog, prior to endodermal cell migration. Inhibition of this regulation prevented cellular migration. These results suggest that Ci-Hox10 regulates endodermal strand cell migration by forming a protrusion and by reconstructing the extracellular matrix.
AB - Hox cluster genes play crucial roles in development of the metazoan antero-posterior axis. Functions of Hox genes in patterning the central nervous system and limb buds are well known. They are also expressed in chordate endodermal tissues, where their roles in endodermal development are still poorly understood. In the invertebrate chordate, Ciona intestinalis, endodermal tissues are in a premature state during the larval stage, and they differentiate into the digestive tract during metamorphosis. In this study, we showed that disruption of a Hox gene, Ci-Hox10, prevented intestinal formation. Ci-Hox10-knock-down larvae displayed defective migration of endodermal strand cells. Formation of a protrusion, which is important for cell migration, was disrupted in these cells. The collagen type IX gene is a downstream target of Ci-Hox10, and is negatively regulated by Ci-Hox10 and a matrix metalloproteinase ortholog, prior to endodermal cell migration. Inhibition of this regulation prevented cellular migration. These results suggest that Ci-Hox10 regulates endodermal strand cell migration by forming a protrusion and by reconstructing the extracellular matrix.
KW - Cell migration
KW - Collagen type IX
KW - Extracellular matrix
KW - Hox
KW - Intestine
KW - Protrusion
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U2 - 10.1016/j.ydbio.2015.03.018
DO - 10.1016/j.ydbio.2015.03.018
M3 - Article
C2 - 25888074
AN - SCOPUS:84931572528
SN - 0012-1606
VL - 403
SP - 43
EP - 56
JO - Developmental Biology
JF - Developmental Biology
IS - 1
ER -