Hypoxia-induced cerebral angiogenesis in mouse cortex with two-photon microscopy

Kazuto Masamoto, Hiroyuki Takuwa, Yutaka Tomita, Haruki Toriumi, Miyuki Unekawa, Junko Taniguchi, Hiroshi Kawaguchi, Yoshiaki Itoh, Norihiro Suzuki, Hiroshi Ito, Iwao Kanno

研究成果: Conference contribution

7 被引用数 (Scopus)


To better understand cellular interactions of the cerebral angiogenesis induced by hypoxia, a spatiotemporal dynamics of cortical microvascular restructuring during an exposure to continuous hypoxia was characterized with in vivo two-photon microscopy in mouse cortex. The mice were prepared with a closed cranial window over the sensory-motor cortex and housed in 8-9 % oxygen room for 2-4 weeks. Before beginning the hypoxic exposure, two-photon imaging of cortical microvasculature was performed, and the follow-up imaging was conducted weekly in the identical locations. We observed that 1-2 weeks after the onset of hypoxic exposure, a sprouting of new vessels appeared from the existing capillaries. An average emergence rate of the new vessel was 15 vessels per unit volume (mm3). The highest emergence rate was found in the cortical depths of 100-200 μm, indicating no spatial uniformity among the cortical layers. Further, a leakage of fluorescent dye (sulforhodamine 101) injected into the bloodstream was not detected, suggesting that the blood-brain barrier (BBB) was maintained. Future studies are needed to elucidate the roles of perivascular cells (e.g., pericyte, microglia, and astroglia) in a process of this hypoxia-induced angiogenesis, such as sprouting, growth, and merger with the existing capillary networks, while maintaining the BBB.

ホスト出版物のタイトルOxygen Transport to Tissue XXXV
出版社Springer New York LLC
出版ステータスPublished - 2013


名前Advances in Experimental Medicine and Biology

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学一般


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