Hzf regulates adipogenesis through translational control of C/EBPα

Hiroyuki Kawagishi; Takeshi Wakoh; Hatsume Uno; Mitsuo Maruyama; Ayako Moriya; Satoru Morikawa; Hideyuki Okano; Charles J. Sherr; Masatoshi Takagi; Masataka Sugimoto

doi:10.1038/emboj.2008.76

Hzf regulates adipogenesis through translational control of C/EBPα

Hiroyuki Kawagishi, Takeshi Wakoh, Hatsume Uno, Mitsuo Maruyama, Ayako Moriya, Satoru Morikawa, Hideyuki Okano, Charles J. Sherr, Masatoshi Takagi, Masataka Sugimoto

研究成果: Article › 査読

24 被引用数 (Scopus)

抄録

Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPα (CCAAT/enhancer- binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPα expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPα is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3′ untranslated region of C/EBPα mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.

本文言語	English
ページ（範囲）	1481-1490
ページ数	10
ジャーナル	EMBO Journal
巻	27
号	10
DOI	https://doi.org/10.1038/emboj.2008.76
出版ステータス	Published - 2008 5月 21

ASJC Scopus subject areas

神経科学（全般）
分子生物学
生化学、遺伝学、分子生物学（全般）
免疫学および微生物学（全般）

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10.1038/emboj.2008.76

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title = "Hzf regulates adipogenesis through translational control of C/EBPα",

abstract = "Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPα (CCAAT/enhancer- binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPα expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPα is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3′ untranslated region of C/EBPα mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.",

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AU - Kawagishi, Hiroyuki

AU - Wakoh, Takeshi

AU - Uno, Hatsume

AU - Maruyama, Mitsuo

AU - Moriya, Ayako

AU - Morikawa, Satoru

AU - Okano, Hideyuki

AU - Sherr, Charles J.

AU - Takagi, Masatoshi

AU - Sugimoto, Masataka

PY - 2008/5/21

Y1 - 2008/5/21

N2 - Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPα (CCAAT/enhancer- binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPα expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPα is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3′ untranslated region of C/EBPα mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.

AB - Adipocyte differentiation requires a well-defined programme of gene expression in which the transcription factor C/EBPα (CCAAT/enhancer- binding protein) has a central function. Here, we show that Hzf (haematopoietic zinc-finger), a previously identified p53 transcriptional target, regulates C/EBPα expression. Hzf is induced during differentiation of preadipocyte cell lines, and its suppression by short hairpin RNA disrupts adipogenesis. In Hzf's absence, expression of C/EBPα is severely impaired because of reduced translation of its mRNA. Hzf physically interacts with the 3′ untranslated region of C/EBPα mRNA to enhance its translation. Taken together, these findings underscore a critical role of Hzf in the adipogenesis regulatory cascade.

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KW - C/EBPα

KW - Hzf

KW - Translation

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Hzf regulates adipogenesis through translational control of C/EBPα

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