@article{5434c58833564901a8cb263fcacf8707,
title = "Icosahedral 60-meric porous structure of designed supramolecular protein nanoparticle TIP60",
abstract = "Supramolecular protein nanoparticles and nanocages have potential in a broad range of applications. Recently, we developed a uniform supramolecular protein nanoparticle, TIP60, symmmetrically self-assembled from fusion proteins of a pentameric Sm-like protein and a dimeric MyoX-coil domain. Herein, we report the icosahedral 60-meric structure of TIP60 solved using single-particle cryo-electron microscopy. Interestingly, the structure revealed 20 regular-triangle-like pores on the surface. TIP60 and its mutants have many modifiable sites on their exterior and interior surfaces. The TIP60 architecture will be useful in the development of biomedical and biochemical nanoparticles/nanocages for future applications.",
author = "Junya Obata and Norifumi Kawakami and Akihisa Tsutsumi and Erika Nasu and Kenji Miyamoto and Masahide Kikkawa and Ryoichi Arai",
note = "Funding Information: We thank Mr Kenji Sasahara at Shinshu University for help in sample preparation and Mr Kazuhiko Nakamura for help in management of the Cryo-EM facility. This work was supported by JSPS KAKENHI Grant numbers JP16K05841, JP17KK0104, JP18K05324, and JP19H02522. This research was supported by Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number JP20am0101115 (support number 1427). We thank Editage (www.editage.com) for language editing. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021.",
year = "2021",
month = oct,
day = "11",
doi = "10.1039/d1cc03114g",
language = "English",
volume = "57",
pages = "10226--10229",
journal = "Chemical Communications",
issn = "1359-7345",
publisher = "Royal Society of Chemistry",
number = "79",
}