Identification of a high-copy-number plasmid suppressor of a lethal phenotype caused by mutant DnaA protein which has decreased intrinsic ATPase activity

Masaki Makise, Kenji Sakamoto, Tomofusa Tsuchiya, Tohru Mizushima

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The induction of a mutant DnaA protein (DnaA E204Q) with decreased intrinsic ATPase activity in cells causes a lethal phenotype. Based on our results that the decreased ATPase activity of DnaA E204Q is activated to the level of a wild-type protein in the presence of partially purified stimulating factors for DnaA ATPase, we tested the hypothesis that genes encoding the stimulating factors can be cloned as high-copy-number plasmid suppressors for the lethality caused by DnaA E204Q. We isolated a number of high copy-number plasmids that suppress the lethal phenotype. The genes responsible for the suppression of the lethal phenotype were revealed to be dnaN, relA, pcnB and cyaA by subcloning, and a site-directed mutational analysis. The mechanisms by which these genes suppressed the lethal phenotype were examined in vivo and in vitro.

本文言語English
ページ(範囲)904-909
ページ数6
ジャーナルBiological and Pharmaceutical Bulletin
22
9
DOI
出版ステータスPublished - 1999 9月

ASJC Scopus subject areas

  • 薬理学
  • 薬科学

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