Imaging of astrocytic activity in living rodents

Norio Takata, Yoshiaki Shinohara, Masamichi Ohkura, Tsuneko Mishima, Junichi Nakai, Hajime Hirase

研究成果: Chapter

4 被引用数 (Scopus)

抄録

Mounting evidence from in vitro experiments supports bidirectional interactions between astrocytes and neurons, suggesting glial involvement in neuronal information processing in the brain. Elevation of the cytosolic calcium ion (Ca2+) concentration has been suggested to be important for gliotransmission; however, the study of Ca2+ dynamics in cerebral cortical astrocytes in vivo became possible only recently. Here, we describe a set of procedures to monitor Ca2+ concentration fluctuations in a population of astrocytes or in astrocytic processes using in vivo two-photon microscopy. Simultaneous recording of neuronal activity by electrodes and astrocytic activity by imaging is a promising way to reveal the nature of neuron-glia interactions in the brain of living rodents.

本文言語English
ホスト出版物のタイトルOptical Imaging of Neocortical Dynamics
出版社Humana Press Inc.
ページ191-207
ページ数17
ISBN(印刷版)9781627037846
DOI
出版ステータスPublished - 2014
外部発表はい

出版物シリーズ

名前Neuromethods
85
ISSN(印刷版)0893-2336
ISSN(電子版)1940-6045

ASJC Scopus subject areas

  • 神経科学一般
  • 生化学、遺伝学、分子生物学一般
  • 薬理学、毒性学および薬学(全般)
  • 精神医学および精神衛生

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