Improved refolding of denatured/reduced lysozyme using disulfide-carrying polymeric microspheres

Hidenobu Shimizu, Keiji Fujimoto, Haruma Kawaguchi

研究成果: Article査読

12 被引用数 (Scopus)


Many proteins tend to aggregate during refolding, especially at high protein concentrations. To reduce aggregation and improve the refolding yields, denatured/reduced lysozyme at 0.5 mg ml-1 was refolded using disulfide-carrying microspheres. The extent of renaturation was evaluated by measuring the content of recovered soluble protein and its enzymatic activity. Addition of the dispersion of the modified microspheres to the aqueous solution of reduced lysozyme resulted in the binding of the proteins on the microsphere surface, followed by the spontaneous release of the bound proteins. As a consequence, a 10% active enzyme was obtained. However, no renaturation occurred without the microspheres due to aggregation. Moreover, when glutathione was added 20 h after incubation with the microspheres, the refolding yields could increase to ~40%. These results indicate that the disulfide moieties of the microspheres play an important role in preventing the aggregation process and catalyzing the refolding reaction through the thiol-disulfide interchange reactions. Copyright (C) 2000 Elsevier Science B.V.

ジャーナルColloids and Surfaces B: Biointerfaces
出版ステータスPublished - 2000 8月

ASJC Scopus subject areas

  • バイオテクノロジー
  • 表面および界面
  • 物理化学および理論化学
  • コロイド化学および表面化学


「Improved refolding of denatured/reduced lysozyme using disulfide-carrying polymeric microspheres」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。