Improved Sendai viral system for reprogramming to naive pluripotency

Akira Kunitomi, Ryoko Hirohata, Vanessa Arreola, Mitsujiro Osawa, Tomoaki M. Kato, Masaki Nomura, Jitsutaro Kawaguchi, Hiroto Hara, Kohji Kusano, Yasuhiro Takashima, Kazutoshi Takahashi, Keiichi Fukuda, Naoko Takasu, Shinya Yamanaka

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Naive human induced pluripotent stem cells (iPSCs) can be generated by reprogramming somatic cells with Sendai virus (SeV) vectors. However, only dermal fibroblasts have been successfully reprogrammed this way, and the process requires culture on feeder cells. Moreover, SeV vectors are highly persistent and inhibit subsequent differentiation of iPSCs. Here, we report a modified SeV vector system to generate transgene-free naive human iPSCs with superior differentiation potential. The modified method can be applied not only to fibroblasts but also to other somatic cell types. SeV vectors disappear quickly at early passages, and this approach enables the generation of naive iPSCs in a feeder-free culture. The naive iPSCs generated by this method show better differentiation to trilineage and extra-embryonic trophectoderm than those derived by conventional methods. This method can expand the application of iPSCs to research on early human development and regenerative medicine.

本文言語English
論文番号100317
ジャーナルCell Reports Methods
2
11
DOI
出版ステータスPublished - 2022 11月 21

ASJC Scopus subject areas

  • バイオテクノロジー
  • 生化学
  • 生化学、遺伝学、分子生物学(その他)
  • 遺伝学
  • 放射線学、核医学およびイメージング
  • コンピュータ サイエンスの応用

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