In vitro precursor microRNA processing assays using Drosophila Schneider-2 cell lysates.

Akira Ishizuka, Kuniaki Saito, Mikiko C. Siomi, Haruhiko Siomi

研究成果: Review article査読

3 被引用数 (Scopus)

抄録

Recent studies have shown that the microRNA (miRNA) pathway is an evolutionarily conserved endogenous pathway that is important for normal development. Mature miRNAs are excised from precursors in a stepwise process and subsequently incorporated into an RNA-induced silencing complex (RISC), which mediates either cleavage of the target messenger RNA (mRNA) or translational repression, depending on the complementarity between the miRNA and its target mRNA. In this chapter, we describe in vitro precursor (pre)-miRNA processing assays using Drosophila Schneider-2 (S2) cell lysates and immunopurified materials.

本文言語English
ページ(範囲)277-286
ページ数10
ジャーナルMethods in molecular biology (Clifton, N.J.)
342
出版ステータスPublished - 2006
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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