Production of a 92-kDa gelatinase / type IV collagenase and tissue inhibitor of metalloproteinases (TIMP) was investigated with human sarcoma cell lines. Among the cytokines and growth factors examined, only human recombinant tumor necrosis factor α (TNFα) induced and stimulated the proteinase with concomitant increase in TIMP expression, but matrix metalloproteinase 2 (72-kDa gelatinase / type IV collagenase) expression was unchanged. These data suggest that gene expression of the two metalloproteinases is regulated in a different fashion and TNFα may be important to allow cancer cells to be more invasive and metastatic.
|Biochemical and Biophysical Research Communications
|Published - 1990 9月 14
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