Inhibition of MMP-2-mediated mast cell invasion by NF-κB inhibitor DHMEQ in mast cells

Naruto Noma, Masataka Asagiri, Masatoshi Takeiri, Saori Ohmae, Kenji Takemoto, Keiko Iwaisako, Nagahiro Minato, Mari Maeda-Yamamoto, Siro Simizu, Kazuo Umezawa

研究成果: Article査読

15 被引用数 (Scopus)


Background: Stimulation with antigen and IgE is known to activate NF-κB in mast cells. In the present research, we studied the role of NF-κB on cellular migration in mast cell-like RBL-2H3 cells and bone marrow-derived mast cells (BMMCs) using the NF-κB inhibitor (-)-DHMEQ. Methods: A Matrigel invasion chamber was used to evaluate cell migration. A PCR array was used to screen the expression of 84 key genes involved in cell migration. Results: (-)-DHMEQ inhibited antigen/IgE-induced NF-κB activation and expressions of its target genes such as IL-6 and TNF-α. (-)-DHMEQ was found to inhibit in vitro invasion toward the antigen without any toxicity. We then looked for NF-κB-dependent genes that would be important for mast cell invasion using the PCR array. (-)-DHMEQ was found to lower the expression of matrix metalloproteinase (MMP)-2. The MMP inhibitor GM6001 also inhibited cellular invasion toward the antigen. These effects of (-)-DHMEQ were obtained in both RBL-2H3 cells and BMMCs. Conclusions: These findings indicate that (-)-DHMEQ suppressed mast cell migration via the inhibition of NF-κB-regulated MMP-2 expression.

ジャーナルInternational Archives of Allergy and Immunology
出版ステータスPublished - 2015 5月 6

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学


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