TY - JOUR
T1 - Intricate recognition of glycolipid-like compounds by HIV-1 envelope proteins evaluated with surface plasmon resonance imaging
AU - Okada, Tomoko
AU - Kimura, Arisa
AU - Miura, Hiroshi
AU - Nishiyama, Toshinori
AU - Mori, Masako
AU - Suzuki, Jyunya
AU - Ogiso, Masayo
AU - Matsuoka, Koji
AU - Sato, Toshinori
AU - Hatanaka, Kenichi
AU - Minoura, Norihiko
N1 - Funding Information:
This work was supported by a grant for “Development of Novel Diagnostic and Medical Applications through Elucidation of Sugar Chain Functions” from the New Energy and Industrial Technology Development Organization (NEDO) of Japan.
PY - 2012/9/1
Y1 - 2012/9/1
N2 - Fusion of human immunodeficiency virus (HIV) to the cell membrane occurs by the specific binding of an envelope protein of HIV-1 (gp120 and gp160) and a glycosphingolipid of the cell membrane. In this study, quantitative and array-based affinity evaluation of gp120 and gp160 was performed by surface plasmon resonance (SPR) and the SPR imaging technique using a substrate immobilized with glycolipid-like compounds (Gb3, GM3, and Lac). Quantitative affinity evaluation showed that gp160 specifically bound to Gb3 and Lac compared with GM3, whereas gp120 showed lower binding affinity and specificity. Array-based evaluation showed that gp160 binds to Gb3 more favorably than Lac and GM3.
AB - Fusion of human immunodeficiency virus (HIV) to the cell membrane occurs by the specific binding of an envelope protein of HIV-1 (gp120 and gp160) and a glycosphingolipid of the cell membrane. In this study, quantitative and array-based affinity evaluation of gp120 and gp160 was performed by surface plasmon resonance (SPR) and the SPR imaging technique using a substrate immobilized with glycolipid-like compounds (Gb3, GM3, and Lac). Quantitative affinity evaluation showed that gp160 specifically bound to Gb3 and Lac compared with GM3, whereas gp120 showed lower binding affinity and specificity. Array-based evaluation showed that gp160 binds to Gb3 more favorably than Lac and GM3.
KW - Carbohydrate
KW - Envelope protein
KW - Glycolipid-like compound
KW - Human immunodeficiency virus (HIV)
KW - Surface plasmon resonance (SPR) imaging
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U2 - 10.1080/07328303.2012.682190
DO - 10.1080/07328303.2012.682190
M3 - Article
AN - SCOPUS:84865965008
SN - 0732-8303
VL - 31
SP - 584
EP - 592
JO - Journal of Carbohydrate Chemistry
JF - Journal of Carbohydrate Chemistry
IS - 7
ER -
