TY - JOUR
T1 - Involvement of tyrosine kinase in growth factor-induced phospholipase C activation in NIH3T3 cells
AU - Imoto, Masaya
AU - Sujikai, Ikuo
AU - Ui, Hideaki
AU - Umezawa, Kazuo
N1 - Funding Information:
This work was partly supportedb y grantsf rom the Ministry of Science, Culture and Education, and the Life ScienceF oundationof Japan.
PY - 1993/2/24
Y1 - 1993/2/24
N2 - Tyrosine kinase inhibitors such as erbstatin and lavendustin derivative inhibited platelet-derived growth factor (PDGF)- and bombesin-induced inositol phosphate formation and phospholipase C (PLC) activation in quiescent NIH3T3 cells. However, bombesin-induced PLC activation was only partially inhibited by tyrosine kinase inhibitors, whereas PDGF-induced activation was completely. Moreover, although bombesin-induced PLC activation was partially inhibited by pertussis toxin alone, this toxin inhibited almost completely in the presence of tyrosine kinase inhibitors. Thus, tyrosine kinase was suggested to be involved in PDGF- and bombesin-induced PLC activation in a different manner.
AB - Tyrosine kinase inhibitors such as erbstatin and lavendustin derivative inhibited platelet-derived growth factor (PDGF)- and bombesin-induced inositol phosphate formation and phospholipase C (PLC) activation in quiescent NIH3T3 cells. However, bombesin-induced PLC activation was only partially inhibited by tyrosine kinase inhibitors, whereas PDGF-induced activation was completely. Moreover, although bombesin-induced PLC activation was partially inhibited by pertussis toxin alone, this toxin inhibited almost completely in the presence of tyrosine kinase inhibitors. Thus, tyrosine kinase was suggested to be involved in PDGF- and bombesin-induced PLC activation in a different manner.
KW - Bombesin
KW - G-protein
KW - Phospholipase C
KW - Platelet-derived growth factor
KW - Tyrosine kinase
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U2 - 10.1016/0005-2760(93)90096-R
DO - 10.1016/0005-2760(93)90096-R
M3 - Article
C2 - 8443236
AN - SCOPUS:0027462745
SN - 0005-2760
VL - 1166
SP - 188
EP - 192
JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
IS - 2-3
ER -