抄録
A family of genes related to the sex-determining region Y gene (SRY) has been termed SOX and the members are known to encode transcriptional factors and be involved in animal development. We have isolated and sequenced a novel Sox cDNA of Xenopus laevis in this study. The cDNA encodes a protein of 309 amino acids, which encompasses a B-type SOX HMG box; the cDNA was named xSox- B1 in this study. The xSox-B1 mRNA is 1.6 kb in length. Various tissues of adult frog were tested for xSox-B1 mRNA by reverse transcription/polymerase chain reaction, and the results showed that xSox-B1 expression was highly restricted to the ovary. The message of the xSox-B1 gene was also detected in unfertilized eggs and the late blastula embryos of X. laevis. Recombinant polypeptide of the xSox-B1 HMG domain preferentially binds to the AACAAT sequence.
| 本文言語 | English |
|---|---|
| ページ（範囲） | 1-6 |
| ページ数 | 6 |
| ジャーナル | Archives of Biochemistry and Biophysics |
| 巻 | 346 |
| 号 | 1 |
| DOI | |
| 出版ステータス | Published - 1997 10月 1 |
| 外部発表 | はい |
ASJC Scopus subject areas
- 生物理学
- 生化学
- 分子生物学