Isolation and identification of hematopoietic stem cell-stimulating substances from kampo (Japanese herbal) medicine, Juzen-Taiho-To

Hiroko Hisha, Haruki Yamada, Masumi H. Sakurai, Hiroaki Kiyohara, Yongan Li, Cheng Ze Yu, Norito Takemoto, Hideki Kawamura, Katsunori Yamaura, Seiichi Shinohara, Yasuhiro Komatsu, Masaki Aburada, Susumu Ikehara

研究成果: Article査読

60 被引用数 (Scopus)

抄録

We have previously found that T J-48 has the capacity to accelerate recovery from hematopoietic injury induced by radiation and the anti-cancer drug mitomycin C (MMC). The effects are found to be due to its stimulation of spleen colony-forming unit (CFU-S) counts on day 14. In the present study, we attempt to isolate and purify the active components in T J-48 extracts using a new in vitro hematopoietic stem cell (HSC) assay method. n-Hexane extract from TJ-48 shows a significant stimulatory activity. The extract is further fractionated by silica gel chromatography and HPLC in order to identify its active components. 1H-NMR and GC-El-MS indicate that the active fraction is composed of free fatty acids (oleic acid and linolenic acid). When 27 kinds of free fatty acids (commercially available) are tested using the HSC proliferating assay, oleic acid, elaidic acid, and linolenic acid are found to have potent activity. The administration of oleic acid to MMC-treated mice enhances CFU-S counts on days 8 and 14 to twice the control group. These findings strongly suggest that fatty acids contained in TJ-48 actively promote the proliferation of HSCs. Although many mechanisms seem to be involved in the stimulation of HSC proliferation, we speculate that at least one of the signals is mediated by stromal cells, rather than any direct interaction with the HSCs.

本文言語English
ページ(範囲)1022-1030
ページ数9
ジャーナルBlood
90
3
DOI
出版ステータスPublished - 1997 8月 1
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 免疫学
  • 血液学
  • 細胞生物学

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