Isolation and transplantation of dopaminergic neurons and neural stem cells

Hideyuki Okano, Takahito Yoshizaki, Takuya Shimazaki, Kazunobu Sawamoto

研究成果: Article査読

20 被引用数 (Scopus)


Although transplantation of mesencephalic tissue is considered a promising therapy for Parkinson's disease (PD), its clinical use is still restricted to a very few cases. A major limiting factor of this therapy is the difficulty of obtaining sufficient quantities of viable embryonic mesencephalic tissue. To overcome this limitation, techniques to produce dopaminergic (DA) neurons in vitro have been developed. However, these cultures are likely to contain a variety of unidentified cells, which must be removed before implantation. Specific cell-surface markers to sort DA neurons or their precursors are not available. We have developed an alternative strategy, by which these cells can be labeled with green fluorescent protein and isolated with fluorescent activated cell sorter. Transplantation of the sorted cells resulted in recovery of a rat model of the PD. This strategy should be useful for developing new therapies for PD.

ジャーナルParkinsonism and Related Disorders
出版ステータスPublished - 2002 10月 1

ASJC Scopus subject areas

  • 神経学
  • 老年医学
  • 臨床神経学


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