@article{b1ad699c46484d0e9fe1909a47ed42a0,
title = "Lactate production is a prioritized feature of adipocyte metabolism",
abstract = "Adipose tissue is essential for whole-body glucose homeostasis, with a primary role in lipid storage. It has been previously observed that lactate production is also an important metabolic feature of adipocytes, but its relationship to adipose and wholebody glucose disposal remains unclear. Therefore, using a combination of metabolic labeling techniques, here we closely examined lactate production of cultured and primary mammalian adipocytes. Insulin treatment increased glucose uptake and conversion to lactate, with the latter responding more to insulin than did other metabolic fates of glucose. However, lactate production did not just serve as a mechanism to dispose of excess glucose, because we also observed that lactate production in adipocytes did not solely depend on glucose availability and even occurred independently of glucose metabolism. This suggests that lactate production is prioritized in adipocytes. Furthermore, knocking down lactate dehydrogenase specifically in the fat body of Drosophila flies lowered circulating lactate and improved whole-body glucose disposal. These results emphasize that lactate production is an additional metabolic role of adipose tissue beyond lipid storage and release.",
author = "Krycer, {James R.} and Quek, {Lake Ee} and Deanne Francis and Fazakerley, {Daniel J.} and Elkington, {Sarah D.} and Alexis Diaz-Vegas and Cooke, {Kristen C.} and Weiss, {Fiona C.} and Xiaowen Duan and Sergey Kurdyukov and Zhou, {Ping Xin} and Tambar, {Uttam K.} and Akiyoshi Hirayama and Satsuki Ikeda and Yushi Kamei and Tomoyoshi Soga and Cooney, {Gregory J.} and James, {David E.}",
note = "Funding Information: 7Supported by Welch Foundation Grant I-1748 and National Institutes of Health Grant R01GM102604. Funding Information: 13Supported by NHMRC Senior Principal Research Fellowship APP1019680 and NHMRC Project Grants GNT1061122 and GNT1086851. To whom cor-respondence may be addressed: Charles Perkins Centre, the University of Sydney, Sydney, New South Wales 2006, Australia. Tel.: 612-8627-1621; E-mail: david.james@sydney.edu.au. Funding Information: This work was supported by the Sydney Informatics Hub, funded by the Uni-versity of Sydney. The authors declare that they have no conflicts of inter-est with the contents of this article. The contents of the published material are solely the responsibility of the authors and do not reflect the views of the National Health and Medical Research Council. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Funding Information: 8Supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grants JP18H04804 and JP18K08219 and by Research on Development of New Drugs (GAPFREE) from the Japan Agency for Medical Research and Development (AMED). Funding Information: 1 Supported by National Health and Medical Research Council (NHMRC) Early Career Fellowship APP1072440, an Australian Diabetes Society Skip Martin Early-Career Fellowship, a Diabetes Australia Research program grant, and a CPC Early-Career Seed Funding grant. Funding Information: 9Supported by the Yamagata prefectural government and the City of Tsuruoka. 10 Supported by AMED-CREST from AMED. 11 Supported by NHMRC Project Grant GNT1086850. Funding Information: Supported by National Health and Medical Research Council (NHMRC) Early Career Fellowship APP1072440, an Australian Diabetes Society Skip Martin Early-Career Fellowship, a Diabetes Australia Research program grant, and a CPC Early-Career Seed Funding grant. 2 Supported by the Judith and David Coffey Fund and Cancer Institute NSW Grant CDF181241. 3 Supported by a CPC Early-Career Seed Funding grant. 4 Present address: Metabolic Research Laboratories, Wellcome Trust-Medical Research Council Institute of Metabolic Science, University of Cambridge, Cambridge, CB2 0QQ, United Kingdom. 5 Supported by University of Sydney-China Scholarship Council Postgraduate Research Scholarship 201606270221. 6 Supported by China Scholarships Council Program Grant 201708410040. 7 Supported by Welch Foundation Grant I-1748 and National Institutes of Health Grant R01GM102604. 8 Supported by Japan Society for the Promotion of Science (JSPS) KAKENHI Grants JP18H04804 and JP18K08219 and by Research on Development of New Drugs (GAPFREE) from the Japan Agency for Medical Research and Development (AMED). 9 Supported by the Yamagata prefectural government and the City of Tsuruoka. 10 Supported by AMED-CREST from AMED. 11 Supported by NHMRC Project Grant GNT1086850. 12 Supported by a Professorial Research Fellowship from the University of Sydney Medical School. Supported by NHMRC Senior Principal Research Fellowship APP1019680 and NHMRC Project Grants GNT1061122 and GNT1086851. Funding Information: 6 Supported by China Scholarships Council Program Grant 201708410040. Funding Information: 2Supported by the Judith and David Coffey Fund and Cancer Institute NSW Grant CDF181241. Publisher Copyright: {\textcopyright} 2020 Krycer et al.",
year = "2020",
month = jan,
day = "3",
doi = "10.1074/jbc.RA119.011178",
language = "English",
volume = "295",
pages = "83--98",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "1",
}