Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain

Hiroyuki Matsumura, Hidetoshi Hasuwa, Naokazu Inoue, Masahito Ikawa, Masaru Okabe

研究成果: Article査読

77 被引用数 (Scopus)

抄録

We have established a transgenic mouse line in which floxed neomycin resistant cassette was inserted between the CAG promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT.

本文言語English
ページ(範囲)275-279
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
321
2
DOI
出版ステータスPublished - 2004 8月 20
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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