TY - JOUR
T1 - Manipulation of Pluripotent Stem Cell Metabolism for Clinical Application
AU - Tohyama, Shugo
AU - Tanosaki, Sho
AU - Someya, Shota
AU - Fujita, Jun
AU - Fukuda, Keiichi
N1 - Funding Information:
Acknowledgements This work was supported by the Highway Program for Realization of Regenerative Medicine from the Japan Science and Technology Agency (to K.F.), the Japanese Heart Foundation research grant (to S. Tohyama) and the SENSHIN Medical Research Foundation (to S. Tohyama).
Funding Information:
This work was supported by the Highway Program for Realization of Regenerative Medicine from the Japan Science and Technology Agency (to K.F.), the Japanese Heart Foundation research grant (to S. Tohyama) and the SENSHIN Medical Research Foundation (to S. Tohyama). Sho Tanosaki and Shota Someya declare that they have no conflict of interest. Shugo Tohyama and Jun Fujita have a patent WO/2016/010165 pending. Keiichi Fukuda has a patent WO/2016/010165 pending, and a patent WO/2007/088874 issued. Keiichi Fukuda is a co-founder of Heartseed Inc.
Publisher Copyright:
© 2017, The Author(s).
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Purpose of review: Pluripotent stem cells (PSCs) have the capacity to differentiate into various types of cells, and are promising cell sources for regenerative therapy and drug screening. However, to realize the clinical application of PSCs, a large number of highly qualified target cells must be stably prepared with low cost. To achieve this, great improvements in the reprogramming, differentiation, and elimination of residual PSCs will be necessary. In this review, we summarize the updated knowledge about metabolism in PSCs and its application. Recent findings: Recent studies have shown that PSCs have distinct metabolic profiles compared to differentiated cells. The metabolic profiles of PSCs are indispensable for the maintenance of pluripotency, self-renewal, differentiation capacity, and cell survival. Summary: Metabolic approaches show improved simplicity, scalability, and lower cost than conventional methods for differentiation and elimination of residual PSCs. Thus, manipulation of PSC metabolism will lead to new technologies to improve their efficiencies.
AB - Purpose of review: Pluripotent stem cells (PSCs) have the capacity to differentiate into various types of cells, and are promising cell sources for regenerative therapy and drug screening. However, to realize the clinical application of PSCs, a large number of highly qualified target cells must be stably prepared with low cost. To achieve this, great improvements in the reprogramming, differentiation, and elimination of residual PSCs will be necessary. In this review, we summarize the updated knowledge about metabolism in PSCs and its application. Recent findings: Recent studies have shown that PSCs have distinct metabolic profiles compared to differentiated cells. The metabolic profiles of PSCs are indispensable for the maintenance of pluripotency, self-renewal, differentiation capacity, and cell survival. Summary: Metabolic approaches show improved simplicity, scalability, and lower cost than conventional methods for differentiation and elimination of residual PSCs. Thus, manipulation of PSC metabolism will lead to new technologies to improve their efficiencies.
KW - Differentiation
KW - Embryonic stem cells
KW - Induced pluripotent stem cells
KW - Metabolism
KW - Purification
KW - Regenerative therapy
UR - http://www.scopus.com/inward/record.url?scp=85042706691&partnerID=8YFLogxK
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U2 - 10.1007/s40778-017-0073-9
DO - 10.1007/s40778-017-0073-9
M3 - Review article
AN - SCOPUS:85042706691
SN - 2198-7866
VL - 3
SP - 28
EP - 34
JO - Current Stem Cell Reports
JF - Current Stem Cell Reports
IS - 1
ER -