Mechanism of the cyanide-catalyzed oxidation of α-ketoaldehydes and α-ketoalcohols

Tadahiko Mashino, Irwin Fridovich

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Cyanide catalyzes the reduction of dioxygen or of ferricytochrome c by dihydroxyacetone phosphate. The rapid initial phase of these reactions, but not the subsequent slow phase, was augmented by incubating the triose phosphate aerobically or anaerobically at pH 9.0 prior to adding the cyanide. The aerobic incubation, which was most effective, was associated with a decline in enediol, whereas the less effective anaerobic incubation was accompanied by an increase in enediol content. This suggested that the α-ketoaldehyde product of autoxidation of the enediol, rather than the enediol itself, was responsible for the rapid phase reaction which followed addition of cyanide. This was confirmed by exploring the cyanide-catalyzed oxidation of the α-ketoaldehyde, phenylglyoxal. The inhibitory effect of the manganese-containing superoxide dismutase indicated that O2- was a kinetically important intermediate of the rapid phase reaction. A reaction mechanism is proposed which is consistent with the results presented.

本文言語English
ページ(範囲)163-170
ページ数8
ジャーナルArchives of Biochemistry and Biophysics
252
1
DOI
出版ステータスPublished - 1987 1月
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学

フィンガープリント

「Mechanism of the cyanide-catalyzed oxidation of α-ketoaldehydes and α-ketoalcohols」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル