Mesenchylmal stem cell culture on poly(N-isopropylacrylamide) hydrogel with repeated thermo-stimulation

Aya Mizutani Akimoto, Erika Hasuike Niitsu, Kenichi Nagase, Teruo Okano, Hideko Kanazawa, Ryo Yoshida

研究成果: Article査読

23 被引用数 (Scopus)


We prepared thermoresponsive hydrogels by mixing poly(N-isopropylacrylamide) (PNIPAAm) derivatives as the main chain components, octa arm polyethylene glycol (PEG) as a crosslinker, and the Arg-Gly-Asp-Ser (RGDS) peptides as cell adhesion units. Human bone marrow derived mesenchymal stem cells (hbmMSCs) were cultured on the hydrogels. The PNIPAAm gel prepared by the post crosslinking gelation method was revealed to be cytocompatible and showed temperature dependent changes in mechanical properties. Repeated changes in the swelling ratio of the PNIPAAm gel affected the shape of the hbmMSCs. With respect to both cytocompatibility and reversibility of changes in mechanical properties, the PNIPAAm gel system could be potentially useful for the analysis of cell mechanobiology.

ジャーナルInternational journal of molecular sciences
出版ステータスPublished - 2018 4月 21

ASJC Scopus subject areas

  • 触媒
  • 分子生物学
  • 分光学
  • コンピュータ サイエンスの応用
  • 物理化学および理論化学
  • 有機化学
  • 無機化学


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