TY - JOUR
T1 - Metabolome analysis based on capillary electrophoresis-mass spectrometry
AU - Hirayama, Akiyoshi
AU - Wakayama, Masataka
AU - Soga, Tomoyoshi
N1 - Funding Information:
This work was supported by the Yamagata Prefectural Government and the City of Tsuruoka, Japan.
Publisher Copyright:
© 2014 Elsevier B.V.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.
AB - Capillary electrophoresis-mass spectrometry (CE-MS) has emerged as a powerful new tool for comprehensive analysis of charged compounds. In this review, we provide a general description of the application of CE-MS in metabolome analysis, including the separation modes of CE, various interfaces and the mass spectrometers used. We also discuss strategies for sample pretreatment, data processing and peak identification, which are important processes in metabolome analysis. In addition, we highlight a number of new techniques to improve metabolite extraction, peak resolution and sensitivity. Finally, we provide some general conclusions and future perspectives.
KW - CE-MS
KW - Capillary electrophoresis
KW - Energy metabolism
KW - Mass spectrometry
KW - Metabolite identification
KW - Metabolome analysis
KW - Metabolomics
KW - Sample pretreatment
KW - Separation
KW - Sheathless interface
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U2 - 10.1016/j.trac.2014.05.005
DO - 10.1016/j.trac.2014.05.005
M3 - Review article
AN - SCOPUS:84907016229
SN - 0165-9936
VL - 61
SP - 215
EP - 222
JO - TrAC - Trends in Analytical Chemistry
JF - TrAC - Trends in Analytical Chemistry
ER -