Microfabricated mobile microplates for handling single adherent cells

Hiroaki Onoe, Shoji Takeuchi

研究成果: Article査読

27 被引用数 (Scopus)

抄録

This paper describes a method for manipulating adherent cells using microfabricated mobile microplates. This method allows us to change the positions of the cells without detaching them from the plates. A variable number of adherent cells ranging from one to a few cells were patterned on microplates (50-75 νm in diameter and 2 νm in thickness) that were fixed to a poly(dimethylsiloxane) (PDMS) sheet. The cell-patterned microplates were released by physical means without the use of chemicals and were manipulated using the flow of the surrounding liquid while the cells were alive. Using this technique, manual handling of two different types of adherent cells, NIH/3T3 and HepG2, was demonstrated in a culture dish. Key advantages of our cell-handling technique using mobile microplates include the ability to move adherent cells as if they were floating cells and to handle multiple numbers of different types of cells on a substrate.

本文言語English
論文番号095003
ジャーナルJournal of Micromechanics and Microengineering
18
9
DOI
出版ステータスPublished - 2008 9月 1
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 材料力学
  • 機械工学
  • 電子工学および電気工学

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