TY - JOUR
T1 - Microfluidic experimental platform for producing size-controlled three-dimensional spheroids
AU - Ota, Hiroki
AU - Miki, Norihisa
N1 - Funding Information:
Hiroki Ota received his BE and ME degrees in applied physics from Keio University in 2005 and 2007, respectively, where he investigated the effect of endothelial permeability on photochemical reactions. Since 2008, he has been a PhD student at Keio University. He was supported by a research fellowship from Japan Society for the Promotion of Science (JSPS) from 2009 to 2011. His principal fields of interest include MEMS-based medical devices, microTAS, and tissue engineering.
Funding Information:
This work was supported in part by a Grant-in-Aid for Scientific Research (S) ( 21226006 ) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and the Scientific Research of Priority Areas, System Cell Engineering by Multi-scale Manipulation . It was also supported in part by Keio University through the Keio Gijuku Fukuzawa Memorial Fund for the Advancement of Education and Research.
PY - 2011/10
Y1 - 2011/10
N2 - We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells were aggregated into chambers arranged in an array by microrotational flow within 120 s to form spheroids. The cell density of the initial medium and hydrodynamic flow in the developed array could be adjusted while keeping the device geometry the same to control spheroid size with a standard deviation of less than 19% of the mean. Using this device, spheroids of HepG2 cells of various size categories could be maintained for three days in the chamber with medium exchange and could be continuously evaluated for topology and hepatic functions. Furthermore, CYP1A1 activities were found to increase with time to a constant level at three days. These results demonstrate that this device is readily applicable to producing and maintaining spheroids for in vitro drug screening and biological research.
AB - We propose a microfluidic experimental platform for producing size-controlled spheroids. Cells were aggregated into chambers arranged in an array by microrotational flow within 120 s to form spheroids. The cell density of the initial medium and hydrodynamic flow in the developed array could be adjusted while keeping the device geometry the same to control spheroid size with a standard deviation of less than 19% of the mean. Using this device, spheroids of HepG2 cells of various size categories could be maintained for three days in the chamber with medium exchange and could be continuously evaluated for topology and hepatic functions. Furthermore, CYP1A1 activities were found to increase with time to a constant level at three days. These results demonstrate that this device is readily applicable to producing and maintaining spheroids for in vitro drug screening and biological research.
KW - Bio MEMS
KW - Microfluidics
KW - Microrotational flow
KW - Spheroid
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U2 - 10.1016/j.sna.2011.03.051
DO - 10.1016/j.sna.2011.03.051
M3 - Article
AN - SCOPUS:80052035105
SN - 0924-4247
VL - 169
SP - 266
EP - 273
JO - Sensors and Actuators, A: Physical
JF - Sensors and Actuators, A: Physical
IS - 2
ER -