Molecular and immunohistochemical analysis of signaling adaptor protein Crk in human cancers

Hiroshi Nishihara, Shinya Tanaka, Masumi Tsuda, Sumie Oikawa, Masae Maeda, Michio Shimizu, Hiroichi Shinomiya, Akira Tanigami, Hirofumi Sawa, Kazuo Nagashima

研究成果: Article査読

58 被引用数 (Scopus)


Crk is a signaling adaptor protein which is mostly composed of SH2 and SH3 domains, and has been shown to play a pivotal role in cell proliferation, differentiation, and migration. Because Crk was originally isolated as an avian sarcoma virus CT10 encoding oncoprotein v-Crk, we examined a potential role for c-Crk in the carcinogenesis of human cancers. First, to analyze gene mutations of c-Crk, we isolated a human bacterial artificial chromosome clone containing Crk genome and exon/intron structures. However, polymerase chain reaction-single strand conformation polymorphism methods failed to show any genomic mutations in the Crk exon which could be related to carcinogenesis. Second, immunohistochemical analysis of c-Crk-II demonstrated that the levels of c-Crk-II were significantly elevated in most of the tumors, particularly in the colon and lung cancers. Furthermore, immunoblot analysis using human lung cancer cell lines revealed that the expression levels of c-Crk-II were correlated to growth rates of cells. The elevated expression levels of c-Crk-II might be related to the development of human cancers.

ジャーナルCancer Letters
出版ステータスPublished - 2002 6月 6

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究


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