Murine homologs of deltex define a novel gene family involved in vertebrate Notch signaling and neurogenesis

Noriyuki Kishi, Zhenyu Tang, Yusuke Maeda, Asami Hirai, Rong Mo, Mikiko Ito, Satoshi Suzuki, Keiko Nakao, Tsutomu Kinoshita, Tom Kadesch, Chi Chung Hui, Spyros Artavanis-Tsakonas, Hideyuki Okano, Kenji Matsuno

研究成果: Article査読

80 被引用数 (Scopus)


Notch signaling plays an important role in cell-fate specification in multicellular organisms by regulating cell-cell communication. The Drosophila deltex gene encodes a modulator of the Notch pathway that has been shown to interact physically with the Ankyrin repeats of Notch. We isolated four distinct cDNAs corresponding to mouse homologs of deltex - mouse Deltex1 (MDTX1), mouse Deltex2 (MDTX2), mouse Deltex2ΔE (MDTX2ΔE), and mouse Deltex3 (MDTX3). Deduced amino acid sequences of these four cDNAs showed a high degree of similarity to Drosophila Deltex and its human homolog, DTX1 throughout their lengths, even though they possess distinct structural features. MDTX proteins formed homotypic and heterotypic multimers. We found that these genes were expressed in the central, peripheral nervous system and in the thymus, overlapping with those of mouse Notch1. In mammalian tissue culture cells, overexpression of any of the four mouse deltex homologs suppressed the transcriptional activity of E47, a basic helix-loop-helix (bHLH) protein, in a manner similar to suppression by an activated form of human Notch1 or human DTX1. In addition, overexpression of MDTX2 and MDTX2ΔE in C2C12 cells under differentiation-inducing conditions suppressed the expression of myogenin, one of the myogenic transcriptional factors; this was also similar to a previously reported activity of constitutively activated Notch. Furthermore, misexpression of any of the MDTX genes in Xenopus embryos resulted in an expansion of the region expressing the neural cell adhesion molecule (N-CAM) gene, a marker for the neuroepithelium. Collectively, our results suggest that these mouse deltex homologs are involved in vertebrate Notch signaling and regulation of neurogenesis.

ジャーナルInternational Journal of Developmental Neuroscience
出版ステータスPublished - 2001 2月 1

ASJC Scopus subject areas

  • 発達神経科学
  • 発生生物学


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