Na+/Mg2+ transporter acts as a Mg2+ buffering mechanism in PC12 cells

Takeshi Kubota, Kentaro Tokuno, Jun Nakagawa, Yoshiichiro Kitamura, Hiroto Ogawa, Yoshio Suzuki, Koji Suzuki, Kotaro Oka

研究成果: Article査読

26 被引用数 (Scopus)


Mg2+ buffering mechanisms in PC12 cells were demonstrated with particular focus on the role of the Na+/Mg2+ transporter by using a newly developed Mg2+ indicator, KMG-20, and also a Na+ indicator, Sodium Green. Carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP), a protonophore, induced a transient increase in the intracellular Mg2+ concentration ([Mg2+]i). The rate of decrease of [Mg2+]i was slower in a Na+-free extracellular medium, suggesting the coupling of Na+ influx and Mg2+ efflux. Na+ influxes were different for normal and imipramine- (a putative inhibitor of the Na+/Mg2+ transporter) containing solutions. FCCP induced a rapid increase in [Na+]i in the normal solution, while the increase was gradual in the imipramine-containing solution. The rate of decrease of [Mg2+]i in the imipramine-containing solution was also slower than that in the normal solution. From these results, we show that the main buffering mechanism for excess Mg2+ depends on the Na+/Mg2+ transporter in PC12 cells.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 2003 3月 28

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


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