New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane; Itsurou Satoh; Naoki Kumagai; Hidetsugu Saito; Akiko Iino; Yasutaka Inagaki; Tetsu Watanabe; Kanji Tsuchimoto; Masaharu Tsuchiya

doi:10.1016/0022-1759(86)90482-5

New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies

Toshio Morizane
, Itsurou Satoh
, Naoki Kumagai
, Hidetsugu Saito
, Akiko Iino
, Yasutaka Inagaki
, Tetsu Watanabe
, Kanji Tsuchimoto
, Masaharu Tsuchiya

研究成果: Article › 査読

2 被引用数 (Scopus)

抄録

A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.

本文言語	English
ページ（範囲）	225-229
ページ数	5
ジャーナル	Journal of Immunological Methods
巻	91
号	2
DOI	https://doi.org/10.1016/0022-1759(86)90482-5
出版ステータス	Published - 1986 7月 24
外部発表	はい

ASJC Scopus subject areas

免疫アレルギー学
免疫学

文献へのアクセス

10.1016/0022-1759(86)90482-5

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引用スタイル

Morizane, T., Satoh, I., Kumagai, N., Saito, H., Iino, A., Inagaki, Y., Watanabe, T., Tsuchimoto, K., & Tsuchiya, M. (1986). New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies. Journal of Immunological Methods, 91(2), 225-229. https://doi.org/10.1016/0022-1759(86)90482-5

Morizane, T, Satoh, I, Kumagai, N, Saito, H, Iino, A, Inagaki, Y, Watanabe, T, Tsuchimoto, K & Tsuchiya, M 1986, 'New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies', Journal of Immunological Methods, vol. 91, no. 2, pp. 225-229. https://doi.org/10.1016/0022-1759(86)90482-5

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title = "New micro-glass-tube leukocyte adherence inhibition assay assessing cell adherence of mononuclear cell subpopulations defined by monoclonal antibodies",

abstract = "A new micro-glass-tube leukocyte adherence inhibition (LAI) assay which is appropriate for detecting delayed type hypersensitivity in vitro has been developed for human leukocytes. Enumeration of adherent cells is replaced by a cellular radioimmunoassay determining antibody binding of the monoclonal reagents, OKT4, OKT8, and OKM1, to glass-adherent cells, fixed by glutaraldehyde or formaldehyde. An LAI reactivity to purified protein derivative of tuberculin (PPD) was detectable in donors giving a positive PPD skin test with OKT4 reagent, but not with the other two reagents.",

keywords = "Delayed-type hypersensitivity, Leukocyte adherence inhibition, Purified protein derivative of tuberculin",

author = "Toshio Morizane and Itsurou Satoh and Naoki Kumagai and Hidetsugu Saito and Akiko Iino and Yasutaka Inagaki and Tetsu Watanabe and Kanji Tsuchimoto and Masaharu Tsuchiya",

note = "Funding Information: This study was supported by a grant from the Ministry of Education, Science and Culture of Japan and by a Grant for the Study of Hepatoma from IBM Japan, Ltd.",

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AU - Iino, Akiko

AU - Inagaki, Yasutaka

AU - Watanabe, Tetsu

AU - Tsuchimoto, Kanji

AU - Tsuchiya, Masaharu

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