@article{bf348fb29b6d4414bbb4125f617e511c,
title = "Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species",
abstract = "Induced pluripotent stem cells (iPSCs) are capable of providing an unlimited source of cells from all three germ layers and germ cells. The derivation and usage of iPSCs from various animal models may facilitate stem cell-based therapy, gene-modified animal production, and evolutionary studies assessing interspecies differences. However, there is a lack of species-wide methods for deriving iPSCs, in particular by means of non-viral and non-transgene-integrating (NTI) approaches. Here, we demonstrate the iPSC derivation from somatic fibroblasts of multiple mammalian species from three different taxonomic orders, including the common marmoset (Callithrix jacchus) in Primates, the dog (Canis lupus familiaris) in Carnivora, and the pig (Sus scrofa) in Cetartiodactyla, by combinatorial usage of chemical compounds and NTI episomal vectors. Interestingly, the fibroblasts temporarily acquired a neural stem cell-like state during the reprogramming. Collectively, our method, robustly applicable to various species, holds a great potential for facilitating stem cell-based research using various animals in Mammalia.",
keywords = "dog, episomal vector, induced pluripotent stem cell, mammalian, marmoset, neural stem cell, pig, primordial germ cell, reprogramming",
author = "Sho Yoshimatsu and Mayutaka Nakajima and Aozora Iguchi and Tsukasa Sanosaka and Tsukika Sato and Mari Nakamura and Ryusuke Nakajima and Eri Arai and Mitsuru Ishikawa and Kent Imaizumi and Hirotaka Watanabe and Junko Okahara and Toshiaki Noce and Yuta Takeda and Erika Sasaki and R{\"u}diger Behr and Kazuya Edamura and Seiji Shiozawa and Hideyuki Okano",
note = "Funding Information: We especially thank Drs. Takashi Sasaki, Jun Kohyama, Ayako Y. Murayama, and Ms. Kanae Ohtsu (at Keio University), Drs. Hirotaka Kondo and Hisashi Shibuya (at Nihon University). We also thank all the laboratory members of H.O. and K.E. for their encouragement and generous support for the current study. This study was performed under the Strategic Research Program for Brain Sciences and Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) of the the AMED (JP20dm0207001 to H.O.), KAKENHI 19J12871 and 20K22660 (to S.Y.), 20H03156 (to K.E.), and internal budgets from Keio University, including the Program for the Advancement of Research in Core Projects on Longevity of the Keio University Global Research Institute from Keio University (to H.O.). Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = apr,
day = "13",
doi = "10.1016/j.stemcr.2021.03.002",
language = "English",
volume = "16",
pages = "754--770",
journal = "Stem cell reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "4",
}