Nongenetic method for purifying stem cell-derived cardiomyocytes

Fumiyuki Hattori; Hao Chen; Hiromi Yamashita; Shugo Tohyama; Yu Suke Satoh; Shinsuke Yuasa; Weizhen Li; Hiroyuki Yamakawa; Tomofumi Tanaka; Takeshi Onitsuka; Kenichiro Shimoji; Yohei Ohno; Toru Egashira; Ruri Kaneda; Mitsushige Murata; Kyoko Hidaka; Takayuki Morisaki; Erika Sasaki; Takeshi Suzuki; Motoaki Sano; Shinji Makino; Shinzo Oikawa; Keiichi Fukuda

doi:10.1038/nmeth.1403

Nongenetic method for purifying stem cell-derived cardiomyocytes

Fumiyuki Hattori, Hao Chen, Hiromi Yamashita, Shugo Tohyama, Yu Suke Satoh, Shinsuke Yuasa, Weizhen Li, Hiroyuki Yamakawa, Tomofumi Tanaka, Takeshi Onitsuka, Kenichiro Shimoji, Yohei Ohno, Toru Egashira, Ruri Kaneda, Mitsushige Murata, Kyoko Hidaka, Takayuki Morisaki, Erika Sasaki, Takeshi Suzuki, Motoaki SanoShinji Makino, Shinzo Oikawa, Keiichi Fukuda

研究成果: Article › 査読

339 被引用数 (Scopus)

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Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.

本文言語	English
ページ（範囲）	61-66
ページ数	6
ジャーナル	Nature Methods
巻	7
号	1
DOI	https://doi.org/10.1038/nmeth.1403
出版ステータス	Published - 2010 1月

ASJC Scopus subject areas

バイオテクノロジー
生化学
分子生物学
細胞生物学

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10.1038/nmeth.1403

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Hattori, F., Chen, H., Yamashita, H., Tohyama, S., Satoh, Y. S., Yuasa, S., Li, W., Yamakawa, H., Tanaka, T., Onitsuka, T., Shimoji, K., Ohno, Y., Egashira, T., Kaneda, R., Murata, M., Hidaka, K., Morisaki, T., Sasaki, E., Suzuki, T., ... Fukuda, K. (2010). Nongenetic method for purifying stem cell-derived cardiomyocytes. Nature Methods, 7(1), 61-66. https://doi.org/10.1038/nmeth.1403

Hattori, F, Chen, H, Yamashita, H, Tohyama, S, Satoh, YS, Yuasa, S, Li, W, Yamakawa, H, Tanaka, T, Onitsuka, T, Shimoji, K, Ohno, Y, Egashira, T, Kaneda, R, Murata, M, Hidaka, K, Morisaki, T, Sasaki, E, Suzuki, T, Sano, M , Makino, S, Oikawa, S & Fukuda, K 2010, 'Nongenetic method for purifying stem cell-derived cardiomyocytes', Nature Methods, vol. 7, no. 1, pp. 61-66. https://doi.org/10.1038/nmeth.1403

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abstract = "Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. A major limitation for cardlomyocyte purification Is the lack of easy and specific cell marking techniques. We found that a fluorescent dye that labels mitochondria, tetramethylrhodamlne methyl ester Perchlorate, could be used to selectively mark embryonic and neonatal rat cardlomyocytes, as well as mouse, marmoset and human PSC-derlved cardlomyocytes, and that the cells could subsequently be enriched (>99% purity) by fluorescence-activated cell sorting. Purified cardlomyocytes transplanted Into testes did not Induce teratoma formation. Moreover, aggregate formation of PSC-derlved cardlomyocytes through homophlllc cell-cell adhesion Improved their survival In the lmmunodefident mouse heart. Our approaches will aid In the future success of using PSC-derlved cardlomyocytes for basic and clinical applications.",

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AU - Suzuki, Takeshi

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AU - Oikawa, Shinzo

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Nongenetic method for purifying stem cell-derived cardiomyocytes

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