Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A. Yoshimura; N. Shudo; S. I. Ikeda; M. Ichikawa; T. Sumizawa; S. I. Akiyama

doi:10.1016/0304-3835(90)90177-Y

Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A. Yoshimura
, N. Shudo
, S. I. Ikeda
, M. Ichikawa
, T. Sumizawa
, S. I. Akiyama

研究成果: Article › 査読

33 被引用数 (Scopus)

抄録

P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

本文言語	English
ページ（範囲）	45-51
ページ数	7
ジャーナル	Cancer Letters
巻	50
号	1
DOI	https://doi.org/10.1016/0304-3835(90)90177-Y
出版ステータス	Published - 1990 4月 9
外部発表	はい

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ASJC Scopus subject areas

腫瘍学
癌研究

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10.1016/0304-3835(90)90177-Y

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abstract = "P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.",

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AU - Yoshimura, A.

AU - Shudo, N.

AU - Ikeda, S. I.

AU - Ichikawa, M.

AU - Sumizawa, T.

AU - Akiyama, S. I.

PY - 1990/4/9

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AB - P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrugresistance (MDR) increased the accumulation of RG in an MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

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KW - multidrug-resistance

KW - resistance modifiers

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Novel screening method for agents that overcome classical multidrug resistance in a human cell line

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UN SDG

ASJC Scopus subject areas

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