Oral intake of α-glucosyl-hesperidin ameliorates selenite-induced cataract formation

Yosuke Nakazawa, Miki Aoki, Sho Ishiwa, Naoki Morishita, Shin Endo, Noriaki Nagai, Naoki Yamamoto, Megumi Funakoshi-Tago, Hiroomi Tamura

研究成果: Article査読

15 被引用数 (Scopus)


Hesperetin is a natural flavonoid with robust antioxidant properties. Our previous study reported that hesperetin can prevent cataract formation. However, an important consideration regarding hesperetin consumption is the limited bioavailability due to its poor solubility. The present study investigated the anti-cataract effects of α-glucosyl hesperidin in vivo and in vitro using a selenite-induced cataract model. SD rats (age, 13 days) were orally administered PBS (0.2 ml) or α-glucosyl hesperidin (200 mg/kg) on days 0, 1 and 2. Sodium selenite was subcutaneously administered to the rats 4 h after the first oral administration on day 0. Antioxidant levels in the lens and blood were measured on day 6. In vitro, human lens epithelial cells were treated with sodium selenite (10 μM) and/or hesperetin (50 or 100 mM) for 24 h and analyzed for apoptosis markers using sub-G1 population and Annexin V-FITC/propidium iodide staining and DNA ladder formation. α-glucosyl hesperidin treatment significantly reduced the severity of selenite-induced cataract. The level of antioxidants was significantly reduced in the selenite-treated rats compared with in the controls; however, they were normalized with α-glucosyl hesperidin treatment. In vitro, hesperetin could significantly reduce the number of cells undergoing apoptosis induced by sodium selenite in human lens epithelial cell lines. Overall, oral consumption of α-glucosyl hesperidin could delay the onset of selenite-induced cataract, at least in part by modulating the selenite-induced cell death in lens epithelial cells.

ジャーナルMolecular Medicine Reports
出版ステータスPublished - 2020

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 遺伝学
  • 腫瘍学
  • 癌研究


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