TY - JOUR
T1 - Orb-weaving spider Araneus ventricosus genome elucidates the spidroin gene catalogue
AU - Kono, Nobuaki
AU - Nakamura, Hiroyuki
AU - Ohtoshi, Rintaro
AU - Moran, Daniel A.Pedrazzoli
AU - Shinohara, Asaka
AU - Yoshida, Yuki
AU - Fujiwara, Masayuki
AU - Mori, Masaru
AU - Tomita, Masaru
AU - Arakawa, Kazuharu
N1 - Funding Information:
The authors thank Akio Tanikawa for morphological identification of spiders, and for helpful comments about phylogenetic discussion along with Akira Shinkai. Hitoshi Kawakami provided photographs of A. ventricosus, and Yuki Takai, Nozomi Abe, and Yuki Onozawa provided technical support in sequencing and proteome analysis. This work was funded by the ImPACT Program of Council for Science, Technology and Innovation (Cabinet Office, Government of Japan) and in part by research funds from the Yamagata Prefectural Government and Tsuruoka City, Japan.
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Members of the family Araneidae are common orb-weaving spiders, and they produce several types of silks throughout their behaviors and lives, from reproduction to foraging. Egg sac, prey capture thread, or dragline silk possesses characteristic mechanical properties, and its variability makes it a highly attractive material for ecological, evolutional, and industrial fields. However, the complete set of constituents of silks produced by a single species is still unclear, and novel spidroin genes as well as other proteins are still being found. Here, we present the first genome in genus Araneus together with the full set of spidroin genes with unamplified long reads and confirmed with transcriptome of the silk glands and proteome analysis of the dragline silk. The catalogue includes the first full length sequence of a paralog of major ampullate spidroin MaSp3, and several spider silk-constituting elements designated SpiCE. Family-wide phylogenomic analysis of Araneidae suggests the relatively recent acquisition of these genes, and multiple-omics analyses demonstrate that these proteins are critical components in the abdominal spidroin gland and dragline silk, contributing to the outstanding mechanical properties of silk in this group of species.
AB - Members of the family Araneidae are common orb-weaving spiders, and they produce several types of silks throughout their behaviors and lives, from reproduction to foraging. Egg sac, prey capture thread, or dragline silk possesses characteristic mechanical properties, and its variability makes it a highly attractive material for ecological, evolutional, and industrial fields. However, the complete set of constituents of silks produced by a single species is still unclear, and novel spidroin genes as well as other proteins are still being found. Here, we present the first genome in genus Araneus together with the full set of spidroin genes with unamplified long reads and confirmed with transcriptome of the silk glands and proteome analysis of the dragline silk. The catalogue includes the first full length sequence of a paralog of major ampullate spidroin MaSp3, and several spider silk-constituting elements designated SpiCE. Family-wide phylogenomic analysis of Araneidae suggests the relatively recent acquisition of these genes, and multiple-omics analyses demonstrate that these proteins are critical components in the abdominal spidroin gland and dragline silk, contributing to the outstanding mechanical properties of silk in this group of species.
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U2 - 10.1038/s41598-019-44775-2
DO - 10.1038/s41598-019-44775-2
M3 - Article
C2 - 31182776
AN - SCOPUS:85065191532
SN - 2045-2322
VL - 9
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 8380
ER -