Osteogenic capillaries orchestrate growth plate-independent ossification of the malleus

Koichi Matsuo, Yukiko Kuroda, Nobuhito Nango, Kouji Shimoda, Yoshiaki Kubota, Masatsugu Ema, Latifa Bakiri, Erwin F. Wagner, Yoshihiro Takeda, Wataru Yashiro, Atsushi Momose

研究成果: Article査読

21 被引用数 (Scopus)


Endochondral ossification is a developmental process by which cartilage is replaced by bone. Terminally differentiated hypertrophic chondrocytes are calcified, vascularized, and removed by chondroclasts before bone matrix is laid down by osteoblasts. In mammals, the malleus is one of three auditory ossicles that transmit vibrations of the tympanic membrane to the inner ear. The malleus is formed from a cartilaginous precursor without growth plate involvement, but little is known about how bones of this type undergo endochondral ossification. Here, we demonstrate that in the processus brevis of the malleus, clusters of osteoblasts surrounding the capillary loop produce bone matrix, causing the volume of the capillary lumen to decrease rapidly in post-weaning mice. Synchrotron X-ray tomographic microscopy revealed a concentric, cylindrical arrangement of osteocyte lacunae along capillaries, indicative of pericapillary bone formation. Moreover, we report that overexpression of Fosl1, which encodes a component of the AP-1 transcription factor complex, in osteoblasts significantly blocked malleal capillary narrowing. These data suggest that osteoblast/endothelial cell interactions control growth plate-free endochondral ossification through ‘osteogenic capillaries’ in a Fosl1-regulated manner.

ジャーナルDevelopment (Cambridge)
出版ステータスPublished - 2015 11月 15

ASJC Scopus subject areas

  • 分子生物学
  • 発生生物学


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