Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest

Megumi Kurita, Hiroaki Suzuki, Hisao Masai, Kiyohisa Mizumoto, Etsuro Ogata, Ikuo Nishimoto, Sadakazu Aiso, Masaaki Matsuoka

研究成果: Article査読

12 被引用数 (Scopus)


Cdc7 expression repressor (CR)/periphilin has been originally cloned as an interactor with periplakin, a precursor of the cornified cell envelope, and suggested to constitute a new type of nuclear matrix. We here show that CR/periphilin is a ubiquitously expressed nuclear protein with speckled distribution. Overexpression of CR/periphilin induces S-phase arrest. Analysis of expression of regulators involved in DNA replication has revealed that both mRNA and protein expression of Cdc7, a regulator of the initiation and continuation of DNA replication, are markedly downregulated by overexpression of CR/periphilin. However, co-expression of Cdc7 only marginally rescues S-phase arrest induced by CR, indicating that CR retards S-phase progression by modifying expression of some genes including Cdc7, which are involved in progression of DNA replication or coordination of DNA replication and S-phase progression.

ジャーナルBiochemical and Biophysical Research Communications
出版ステータスPublished - 2004 11月 12

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学


「Overexpression of CR/periphilin downregulates Cdc7 expression and induces S-phase arrest」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。